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Blood samples from chicken, cow, crested pigeon and sleepy lizard had been spotted onto FTA® paper as normal. The blood samples from the hen and cow contained anticoagulant . As anticipated, observable amounts of DNA were solely observed from the 1 mm discs from species which have nucleated pink blood cells although only small quantities of DNA had been observed from frogs. Observable quantities of DNA have been eluted from 5 mm punches from all species, nucleated or not, from blood samples and other tissues.